RELION

Software name: 
RELION
Policy 

RELION is freely available to users at HPC2N. 

General 

RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM).

Description 

RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology.

Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB.

A more detailed description of its implementation is given in Scheres (2012) JSB. If RELION is useful in your work, please cite at least one of these papers.

Availability 

On HPC2N we have RELION available as a module on Kebnekaise.

Usage at HPC2N 

To use the RELION module, first add it to your environment. Use:

module spider relion

to see which versions are available and how to load the module and the needed prerequisites.

Example, loading RELION version 2.0.3

ml GCC/6.3.0-2.27
ml OpenMPI/2.0.2
ml RELION/2.0.3 

You can read more about loading modules on our Accessing software with Lmod page and our Using modules (Lmod) page.

Additional info 

More information about RELION and how to run it can be found on the RELION Homepage.

Updated: 2017-09-21, 11:05